Exam 2--Winter 2004 practice

Exam 2--Winter 2004 practice - Bio 305 Genetics Hour Exam#2...

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Bio 305 Genetics Hour Exam #2 Special Code 000000 1 INSTRUCTIONS 1. THE ANSWER SHEET WILL NOT BE SCORABLE UNLESS YOU USE A #2 PENCIL TO FILL IT OUT. IF YOU DO NOT HAVE A #2 PENCIL, GET ONE FROM AN INSTRUCTOR. 2. BEFORE BEGINNING THE EXAM, FILL OUT THE INFORMATIONAL SECTIONS ON THE ACCOMPANYING ANSWER SHEET COMPLETELY READ AND FOLLOW THE INSTRUCTIONS ON THE FORM EXACTLY GRID IN YOUR NAME, STUDENT I.D. #, SECTION #, AND SPECIAL CODE # ON THE LEFT-HAND PORTION OF THE ANSWER SHEET. BE SURE TO FILL IN THE CIRCLE IN THE "SPECIAL CODE" COLUMN THAT CORRESPONDS TO THE SPECIAL CODE # THAT APPEARS AT THE TOP OF THE EXAM PAGE. BE VERY NEAT AND CAREFUL WHEN FILLING OUT THE ANSWER SHEET. THE SCANNER IS SENSITIVE AND MAY INTERPRET STRAY PENCIL MARKS AS ANSWERS. 3. EACH QUESTION HAS ONLY ONE CORRECT (OR BEST) ANSWER; ONLY ONE CIRCLE PER QUESTION SHOULD BE FILLED. 4. WHEN YOU FINISH, HAND YOUR ANSWER SHEET TO AN INSTRUCTOR PERSONALLY . DO NOT GIVE IT TO ANYONE ELSE TO HAND IN. ONLY ONE ANSWER SHEET PER STUDENT WILL BE ACCEPTED. 5. THE EXAM CONSISTS OF 20 QUESTIONS PLUS THREE BONUS QUESTIONS AND THREE BLANK PAGES. THE BLANKS ARE FOR YOUR USE AS SCRATCH PAPER. SUGGESTION : GO THROUGH THE EXAM, CIRCLING THE ANSWERS ON THE EXAM ITSELF (YOU CAN USE ANY PEN OR PENCIL FOR THAT). WAIT TO FILL IN THE CIRCLES ON THE ANSWER SHEET WITH A #2 PENCIL UNTIL YOU HAVE FINISHED THE EXAM AND REVIEWED YOUR CHOICES, OR UNTIL THE LAST 15 MINUTES OF THE EXAM PERIOD.
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Bio 305 Genetics Hour Exam #2 Special Code 000000 2 (1) There are at least two genes required for argnine biosynthesis in E. coli, argA + and argB + . You have isolated several new arg strains of E. coli. You want to determine whether these mutations are allelic with the known argB + gene. Which of the following crosses would allow you to make this distinction? Note: leu strains require leucine for growth; azi R strains can grow in the presence of azide while azi S strains cannot. Strains to cross Assess growth on minimal medium plus: (a) leu ; azi S ; F' (azi R ; argA + ) × leu + ; arg azide (b) leu ; azi S ; F' (azi R ; argA + ) × leu + ; arg leucine (c) leu ; azi S ; F' (azi R ; argB + ) × leu + ; arg azide (d) leu ; azi S ; F' (azi R ; argB + ) × leu + ; arg leucine (e) leu ; azi R ; F' (azi S ; argA + ) × leu + ; arg azide (f) leu ; azi R ; F' (azi S ; argB + ) × leu + ; arg azide (g) leu + ; azi S ; F' (azi R ; argA + ) × leu ; arg azide (h) leu + ; azi S ;F' (azi R ; argA + ) × leu ; arg leucine (i) leu + ; azi S ; F' (azi R ; argB + ) × leu ; arg azide (j) leu + ; azi S ; F' (azi R ; argB + ) × leu ; arg leucine ______________________________________________________________________________ (2) In the snail Limnia , the direction that the shell coils is determined by a strict maternal effect gene: the s + allele is dominant and causes right-handed coiling, while the s - allele is recessive and causes left-handed coiling. You cross a right-handed male with a right-handed female, and observe that all of the F 1 progeny are right-handed coilers. You test each of the F 1 females by crossing them with right-handed s + s + males, and observe the following kinds of broods: 31 F1 females 100% right-handed progeny 28 F1 females 100% left-handed progeny What were the genotypes of the P 0 female and male?
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