Sept 13 - Biochem 423 Robert A Orlando Ph.D ENZYMES –...

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Unformatted text preview: Biochem 423 Robert A. Orlando, Ph.D. ENZYMES – REGULATION 4 MAJOR TYPES OF REGULATION – 1) IRREVERSIBLE INHIBITOR – “SUICIDE INHIBITOR” TRAPS ENZYME IN “ES” STATE SUBSTRATE ANALOG – TARGETS CATALYTIC SITE 2) COMPETITIVE INHIBITOR – REVERSIBLE 54 | P a g e Biochem 423 Robert A. Orlando, Ph.D. LINEWEAVER‐BURK ANALYSIS OF COMPETITIVE INHIBITOR 3) NON‐COMPETITIVE INHIBITOR – REVERSIBLE 55 | P a g e Biochem 423 Robert A. Orlando, Ph.D. LINEWEAVER‐BURK ANALYSIS OF NON‐COMPETITIVE INHIBITOR 4) COVALENT MODIFICATION – REVERSIBLE INHIBIT OR ACTIVATE TYPICALLY BY PHOSPHORYLATION Can have multiple phosphorylations on one protein for increased level of regulation. 56 | P a g e Biochem 423 Robert A. Orlando, Ph.D. ALLOSTERIC REGULATION – INDUCES CONFORMATIONAL CHANGE TYPICALLY FOR MULTI‐SUBUNIT PROTEINS INHIBITION CURVE → SIGMOIDAL → INDICATING SUBUNIT COOPERATIVITY Decreasing Km Higher affinity for substrate Increasing Km Lower affinity for substrate 57 | P a g e ...
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