Lesson%204%20find%20correct%20reading%20frame%20for%20each%20exon

Lesson%204%20find%20correct%20reading%20frame%20for%20each%20exon

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Vector NTI  Lesson 4 Find the reading frames for each  exon in your gene of interest
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Go to wormbase.org and type in the gene name, in this case C49G7.11, or djr- 1.2 and click on ‘Nucleotides (coding/transcript)’
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Triple click on the first row of the ‘ spliced  coding region’ and copy the region  and paste in word.
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Combine into complete exons as show below Now copy the first exon shown in yellow and go to Vector NTI.   Sometimes when you  think you are selecting the entire it is only the first row.  Make sure it is a continuous line of text  by deleting spaces between rows.  Make sure you do  NOT  delete and part of the sequence.
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Click on ‘Edit’ > ‘Find Sequence’ and paste the sequence and click ‘find next’ ATG – start codon
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Now translate the found sequence and verify that this matches the predicted 
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Unformatted text preview: protein from lesson 3 Then repeat for all the exons in your gene Please note that all the exons are not perfect spliced so that when they are translated it might not match the predicted amino acid sequence Here is the second exon and note there is not a single stop codon, indicated by periods or dots Notice that the reading frame shifted from +2 to +1. This is why you cannot simply highlight the entire gene and translate and expect to see your protein n exon 3 it is reading frame +3 that is translated and notice the stop codon. Match up the amino acids here to what is predicted in the other window in Vector N You must do this for your gene of interest! This is one of the hardest assignments, if you understand this you are doing great!...
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