Biotechnology_ - Biotechnology! E.coliandMolecular Biology....

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Biotechnology!!
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E. coli  and Molecular  Biology. Biotechnology !
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Plasmids Characteristics are _______
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What is the purpose of the ORI, marker, and  MCS What is a Transformation?
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Genetic Engineering
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What is Ligation? What is amp short for? What is selection?
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Organisms used in Biotechnology Bacteria Viruses Animal cells Insect cells Yeast Fungi Plants
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Bacteria Particularly  E. coli  and certain bacilli –  1 st  biopharmaceutical made in 1982 Advantages: Served as a model system for years Well characterized High levels of heterologous proteins can be achieved Grow rapidly and in simple inexpensive media Fermentation technology is well established Disadvantages Inability to do post-translational modifications Cannot splice introns Lipopolysaccharide on the surface Intracellular accumulation of protein
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Eukaryotic cell systems Particularly cell systems such as CHO and BHK 1
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This note was uploaded on 12/16/2010 for the course BIOL 1010 taught by Professor Anafi/quinlan during the Spring '08 term at York University.

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Biotechnology_ - Biotechnology! E.coliandMolecular Biology....

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