Biotechnology_ - Biotechnology E.coliandMolecular Biology...

Info icon This preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
Biotechnology!!
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
E. coli  and Molecular  Biology. Biotechnology !
Image of page 2
Plasmids Characteristics are _______
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
What is the purpose of the ORI, marker, and  MCS What is a Transformation?
Image of page 4
Genetic Engineering
Image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
What is Ligation? What is amp short for? What is selection?
Image of page 6
Organisms used in Biotechnology Bacteria Viruses Animal cells Insect cells Yeast Fungi Plants
Image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Bacteria Particularly  E. coli  and certain bacilli –  1 st  biopharmaceutical made in 1982 Advantages: Served as a model system for years Well characterized High levels of heterologous proteins can be achieved Grow rapidly and in simple inexpensive media Fermentation technology is well established Disadvantages Inability to do post-translational modifications Cannot splice introns Lipopolysaccharide on the surface Intracellular accumulation of protein
Image of page 8
Eukaryotic cell systems
Image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern