Lecture+13 (1)

Lecture+13 (1) - BIS101002 11/16/10 Lecture13:

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BIS101-002 11/16/10 Lecture 13: Regulation of gene expression in bacteria
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LECTURE 13: OUTLINE Gene regulation in bacteria: Transcription and operons The lac operon, and regulatory mutations Catabolite repression of the lac operon
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LECTURE 13: OUTLINE Gene regulation in bacteria: Transcription and operons The lac operon, and regulatory mutations Catabolite repression of the lac operon
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Transcriptional regulation in bacteria:  Positive and Negative Control
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Transcriptional regulation in bacteria:  Positive and Negative Control
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Prokaryote activators and repressors often  exist in two conformational states
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Operons: genes of related function transcribed in a  single mRNA (and thus co-regulated)
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LECTURE 13: OUTLINE Gene regulation in bacteria: Transcription and operons The lac operon, and regulatory mutations Catabolite repression of the lac operon
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Lac Operon Encodes 3 Proteins Hydrolyzes lactose into glucose  and galactose Transports  lactose into  the cell Function  not known
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Β -galactosidase hydrolyzes lactose into glucose and  galactose
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Lac Operon Encodes 3 Proteins Hydrolyzes lactose into glucose  and galactose Transports  lactose into  the cell Function  not known
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Lac Operon Encodes 3 Proteins Hydrolyzes lactose into glucose  and galactose Transports  lactose into  the cell Function  not known
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Lac Operon Encodes 3 Proteins Hydrolyzes lactose into glucose  and galactose Transports  lactose into  the cell Function  not known
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Transcriptional regulation of the lac operon
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Transcriptional regulation of the lac operon
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Jacob and Monod used genetics to study gene  regulation APOG!!
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IPTG is an artificial inducer of the lac operon - enters the cell without permease lacY - doesn’t need to be converted by lacZ (unlike lactose)
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Partial diploids allow complementation experiments in  bacteria imprecise excision: an integrated F factor forms an extrachromosomal plasmid that includes host genes F’ with lac operon can be transferred to recipient by conjugation (recipient becomes merodiploid) 5-18
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Classes of Regulatory Mutants Phenotypes: Constitutive Non-inducible
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Phenotypes of Mutants in Positive and Negative  Regulation Is this an example of a: a)Constitutive mutation b)Non-inducible mutation X
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Phenotypes of Mutants in Positive and Negative  Regulation Is this an example of a: a)Constitutive mutation a)Non-inducible mutation X
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Phenotypes of Mutants in Positive and Negative  Regulation Is this an example of a: a)Constitutive mutation b)Non-inducible mutation X
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Phenotypes of Mutants in Positive and Negative  Regulation Is this an example of a: a)Constitutive mutation a)Non-inducible mutation X
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partial diploid with Z-? A)Recessive
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This note was uploaded on 12/17/2010 for the course BIS 101 taught by Professor Simonchan during the Fall '08 term at UC Davis.

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Lecture+13 (1) - BIS101002 11/16/10 Lecture13:

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