Lecture2 - BCH 227 FALL 2010 LECTURE 2: THERMODYNAMICS 1...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Thermodynamics BIOCHEM 227 SUMMER 2010 Lecture 2 Wednesday, September 1, 2010 TODAY • Continue: example from last lecture • Chemical elements in biochemistry • Review of functional groups • Review of basic thermodynamics BCH 227 FALL 2010 LECTURE 2: THERMODYNAMICS 1 SEP 2010
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 An Example: Enzyme Design Nature 453 : 190 (2008) BCH 227 FALL 2010 LECTURE 2: THERMODYNAMICS 1 SEP 2010
Background image of page 2
3 Enzyme Design Example • Design an enzyme to carry out the Kemp elimination – abstract a proton via a general base – no natural enzyme for this reaction •How ? –Asp or G lu s idecha in – His positioned by Asp BCH 227 FALL 2010 LECTURE 2: THERMODYNAMICS 1 SEP 2010
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
4 Enzyme Design Example • Design parameters – structure: catalytic residues, other residues –pKa – quantum calculations – need a protein scaffold • Result: functional catalysts for both designs – Activity confirmed by assays – Structure confirmed by crystallography Enzyme Design Example BCH 227 FALL 2010 LECTURE 2: THERMODYNAMICS 1 SEP 2010
Background image of page 4
5 • Comparison of design (grey) and actual (cyan): Enzyme Design Example BCH 227 FALL 2010 LECTURE 2: THERMODYNAMICS 1 SEP 2010
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 6
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 19

Lecture2 - BCH 227 FALL 2010 LECTURE 2: THERMODYNAMICS 1...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online