Lecture5 - BCH 227 FALL 2010 LECTURE 5: PROTEIN...

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1 Protein Experimental Techniques BIOCHEM 227 FALL 2010 Lecture 5 Wednesday, September 8, 2010 TODAY • A few more words on disulfides • Protein experimental methods – Overexpression – Purification – Gel electrophoresis – Structure determination BCH 227 FALL 2010 LECTURE 5: PROTEIN EXPERIMENTAL TECHNIQUES 6 SEP 2010
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2 Primary Structure - Disulfides Protein Experimental Methods BCH 227 FALL 2010 LECTURE 5: PROTEIN EXPERIMENTAL TECHNIQUES 6 SEP 2010
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3 How Do We Study Proteins? • Need a source of protein • Need to extract/purify it • Need to measure quantities • Need to determine structure • Need to examine function BCH 227 FALL 2010 LECTURE 5: PROTEIN EXPERIMENTAL TECHNIQUES 6 SEP 2010
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4 Sources of Protein • Historical: large cultures, homogenization of tissue • Today: overexpression, “cell-free systems” Purification • Cell lysis • Centrifugation • Historical: batch precipitation •Ch rom a tog r
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5 Chromatography BCH 227 FALL 2010 LECTURE 5: PROTEIN EXPERIMENTAL TECHNIQUES 6 SEP 2010
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6 Ion-Exchange Gel Filtration BCH 227 FALL 2010 LECTURE 5: PROTEIN EXPERIMENTAL TECHNIQUES 6 SEP 2010
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This note was uploaded on 12/19/2010 for the course BIOCHEM 227 taught by Professor Coggins during the Fall '10 term at Duke.

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Lecture5 - BCH 227 FALL 2010 LECTURE 5: PROTEIN...

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