Lecture9 - BCH 227 FALL 2010 LECTURE 9: ENZYMATIC CATALYSIS...

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1 Enzymatic Catalysis Michaelis-Menten Kinetics BIOCHEM 227 FALL 2010 Lecture 9 Friday, September 17, 2010 TODAY • A brief word on folding • Types of enzymatic catalysis • Michaelis-Menten kinetics BCH 227 FALL 2010 LECTURE 9: ENZYMATIC CATALYSIS & MICHAELIS-MENTEN KINETICS 17 SEP 2010
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2 Folding Enzymatic Catalysis BCH 227 FALL 2010 LECTURE 9: ENZYMATIC CATALYSIS & MICHAELIS-MENTEN KINETICS 17 SEP 2010
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3 Types of Catalysis • General acid/base • Nucleophilic • Electrophilic General Acid/Base Catalysis • A group on the enzyme donates/accepts a proton O H - BE n z O n z H BCH 227 FALL 2010 LECTURE 9: ENZYMATIC CATALYSIS & MICHAELIS-MENTEN KINETICS 17 SEP 2010
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4 General Acid/Base Catalysis • Example: isomerization HO H O Enz B - HA Enz B - Enz General Acid/Base Catalysis • Example: isomerization BCH 227 FALL 2010 LECTURE 9: ENZYMATIC CATALYSIS & MICHAELIS-MENTEN KINETICS 17 SEP 2010
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5 General Acid/Base Catalysis • Example: isomerization General Acid/Base Catalysis • Example: isomerization BCH 227 FALL 2010 LECTURE 9: ENZYMATIC CATALYSIS & MICHAELIS-MENTEN KINETICS 17 SEP 2010
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6 General Acid/Base Catalysis Nucleophilic Catalysis • A group on the enzyme acts as a nucleophile BCH 227 FALL 2010 LECTURE 9: ENZYMATIC CATALYSIS & MICHAELIS-MENTEN KINETICS 17 SEP 2010
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7 Electrophilic Catalysis • A group in the enzyme serves as an electrophile , pulling electrons to one side of the substrate • Allows protons to be abstracted from carbon • Three types: –meta l ions – Lys sidechains (formation of imine) –cofactors ( e.g. Vitamin B 6 ) Electrophilic Catalysis • Metal ions as electrophiles BCH 227 FALL 2010 LECTURE 9: ENZYMATIC CATALYSIS & MICHAELIS-MENTEN KINETICS 17 SEP 2010
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8 Electrophilic Catalysis • Imines/iminium cations Electrophilic Catalysis • Formation of eneamines from iminiums BCH 227 FALL 2010 LECTURE 9: ENZYMATIC CATALYSIS & MICHAELIS-MENTEN KINETICS 17 SEP 2010
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Lecture9 - BCH 227 FALL 2010 LECTURE 9: ENZYMATIC CATALYSIS...

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