Lecture10 - BCH 227 FALL 2010 LECTURE 10 MORE KINETICS 20...

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1 More Kinetics BIOCHEM 227 FALL 2010 Lecture 10 Monday, September 20, 2010 TODAY Correction to bad mechanism from Fri • Continued: Michaelis-Menten kinetics Some more advanced examples BCH 227 FALL 2010 LECTURE 10: MORE KINETICS 20 SEP 2010
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2 H O Enz B - HA Enz B - Enz H OH General Acid/Base Catalysis • Example: isomerization H O Enz B - HA Enz B - Enz H OH General Acid/Base Catalysis • Example: isomerization BCH 227 FALL 2010 LECTURE 10: MORE KINETICS 20 SEP 2010
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3 General Acid/Base Catalysis • Example: isomerization General Acid/Base Catalysis • Example: isomerization BCH 227 FALL 2010 LECTURE 10: MORE KINETICS 20 SEP 2010
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4 Michaelis-Menten Kinetics Michaelis-Menten Kinetics E + S R k 1 k 1 ES R k 2 k 2 E + P For this model… – assume only one substrate – assume ES → E + P is the rate-limiting step so we don’t see EP… BCH 227 FALL 2010 LECTURE 10: MORE KINETICS 20 SEP 2010
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5 Michaelis-Menten Kinetics E + S R k 1 k 1 ES k cat E + P Initial rate assumption – assume we start with no P, and that it builds up slowly – we are observing the “initial rate” – no reverse reaction is observed k cat is rate of catalytic step Michaelis-Menten Kinetics E + S R k 1 k 1 ES k cat E + P Assume [S] >> [E] – [S] will change very slowly – fewer variables are changing simultaneously! – also, this is a more common experimental condition BCH 227 FALL 2010 LECTURE 10: MORE KINETICS 20 SEP 2010
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6 Michaelis-Menten Kinetics E + S R k 1 k 1 ES k cat E + P Rate of reaction will be v = k cat [ES] Michaelis-Menten Kinetics E + S R k 1 k 1 ES k cat E + P Let [E] 0 be the total enzyme [E] 0 = [E] + [ES] BCH 227 FALL 2010 LECTURE 10: MORE KINETICS 20 SEP 2010
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