Lecture11 - BCH 227 FALL 2010 LECTURE 11: STRUCTURE AND...

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1 Structure and Function in Enzymes BIOCHEM 227 FALL 2010 Lecture 11 Wednesday, September 22, 2010 TODAY • A few more words on Michaelis-Menten • Enzyme inhibition • Structure and function in enzymes –Chymotryps in –Eno lase –LpxC –A ldo lase
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2 Michaelis-Menten Kinetics Michaelis-Menten Kinetics •I f k cat << k -1 , K M is the dissociation constant for ES formation –h igher K M = less effective binding – other cases: apparent dissocation constant cat 1 M 1 kk K k + = BCH 227 FALL 2010 LECTURE 11: STRUCTURE AND FUNCTION IN ENZYMES
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3 Inhibition • Adding an additional molecule, which reduces catalytic activity of an enzyme in vivo –pharmaceut ica ls – research tool for studying enzymes Reversible Inhibition • Noncovalent inhibitors are reversible – they can come back off BCH 227 FALL 2010 LECTURE 11:
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4 Reversible Inhibition Competitive inhibition – inhibitor competes with substrate for access to the active site –a lters K M Reversible Inhibition Uncompetitive inhibition – inhibitor binds to ES complex –a lters V max and K M BCH 227 FALL 2010 LECTURE 11:
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5 Reversible Inhibition Mixed inhibition – inhibitor binds to E or ES – also alters both V max and K M Structure and Function in Enzymes BCH 227 FALL 2010 LECTURE 11: STRUCTURE AND FUNCTION IN ENZYMES 22 SEP 2010
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Lecture11 - BCH 227 FALL 2010 LECTURE 11: STRUCTURE AND...

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