Structure of a bacterial ribonuclease P holoenzyme in

Structure of a bacterial ribonuclease P holoenzyme in -...

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Structure of a bacterial ribonuclease P holoenzyme in complex with tRNA Wang Bing 2010-11-23
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An ancient RNA-based world
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Structures of RNase P Crystal structure of the specificity domain of ribonuclease P (2003) Crystal structure of the RNA component of bacterial ribonuclease P (2005) Structure of ribonuclease P-a universal ribozyme (2006) Structure of a bacterial ribonuclease P holoenzyme in complex with tRNA (2010)
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Holoenzyme purification and enzymatic assay Purification P RNA, tRNA, P protein independently. Form the RNase P holoenzyme for crystallization.
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Data collection
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Overall structure of the holoenzyme in
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Unformatted text preview: complex with tRNA tRNA recognition by RNase P is mediated by RNA-RNA interaction Protein–RNA contacts within the RNase P holoenzyme Pre-tRNA leader–protein interactions in the RNase P holoenzyme Structure of the RNase P active site environment Mechanistic implications • Substrate specificity Highly conserved regions of tRNA and P RNA involved in tRNA recognition The protein cofactor binding with the 5’ leader pre-tRNA substrate • Chemical mechanism Metals play a directo role in catalyzing A transition state model Who are “more prone” to fake research? THANK YOU...
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