Unformatted text preview: concentration. 3 Part 4 – determination of the iron content in coordination compound sample. 1. Add 10% hydroxylami ne s ADD 2mL of hydroxylamine instead of 1 mL in step 1. 2. Add sodium acetate 3. Add 8 mL 0.3% 1,10 phenanthroline and mix 4. Add 0.3% 1,10 phenanthroline to mark on flask and mix well! 5. Measure and record the absorbance 6. You want an absorbance reading that is between your lowest and highest standard. (Absorbance between 0.1 and 1.0) 4 7. If it’s too high, do another dilution and test it again. s RECORD ALL DILUTIONS ! s RECORD ALL MEASURED ABSORBANCE VALUES ! 8. Make all of your dilutions 5 ml to 25 ml. 9. Do your reading in triplicate – that is, use three different samples. a) They can be the same concentration that you use to measure three times b) Or you may use three different concentrations. 5...
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- Spring '08
- Chemistry, 5 mL, Trademark dilution, phenanthroline