cross II report

cross II report - Drosophila Cross II lab report Objective...

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Drosophila Cross II lab report Objective The purpose of this experiment was to determine the order of selected traits of Drosophila melanogaster and to create a composite genetic map from the experimental data found to be compared to the scientifically accepted data already in existence. Materials and Methods In week one, three crosses were set up. In Cross A, yellow crossveinless were crossed with white. In Cross B, yellow were crossed with miniature forked. In Cross C, miniature were crossed with yellow crossveinless. Virgin females were used for all crosses. Four traits were selected for this cross: yellow, which refers to a yellow body color; crossveinless, which refers to a lack of crossveins in the wings; miniature, which refers to the size of he wings; white, which refers to the color of the eyes. These crosses were the P1 generation. These crosses were then incubated until week two. In week two, the parents were anesthetized using carbon dioxide, removed from the vial and disposed of. This step ensures that the parents are excluded from the final data count as they are not the product of the experiment. The crosses consisting of eggs and larvae were left to continue to incubate until the following week. In week three, the flies were again anesthetized using carbon dioxide so that they could be counted and classified into sex and phenotype. The F1 x F1 vials were also set up. For these vials, virgin females were not required. Week four followed the procedure week two. The parents were anesthetized using carbon dioxide, removed from the vial and disposed of. The eggs and larvae were left undisturbed to continue the cross. They were allowed to incubate until the following final
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