Lab Quiz 7 Questions Bio2203 Spring 2008

Lab Quiz 7 Questions Bio2203 Spring 2008 - Quiz Question...

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Quiz Question Pool 7 Spring 2008 Below please find the seventh of the weekly lab quizzes. This week the ENTIRE quiz will be drawn from this list of questions. The total point value for each quiz will be 10 points. Questions on phage recombination : 1) What does chloroform do? (2 points) 2) What does KCN do? (2 points) 3) How many different genes are we following in this experiment? How many alleles are there of each? (3 points) 4) There are 4 quantities that you must measure in order to work out the recombination frequency between the two mutations. What are they? (5 points) 5) a. What is multiplicity of infection (MOI)? (1 point) b. What is the ratio of phage to bacteria that you are combining in step 1 page 69? SHOW YOUR WORK! (2 points) c. Why is this ratio desirable for our purposes? (2 points) 6) Why must you multiply the “titer on K” by 2 to get the recombination frequency? (3 points) 7) By what 2 different mechanisms could mutant phages change to become capable of lysing E. coli K? (2 points)
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This lab report was uploaded on 04/04/2008 for the course BIO 2203 taught by Professor Athwal/haines during the Spring '08 term at Temple.

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Lab Quiz 7 Questions Bio2203 Spring 2008 - Quiz Question...

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