Lab Quiz 8 Questions Bio2203 Spring 2008

Lab Quiz 8 Questions Bio2203 Spring 2008 - c. In fact,...

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Quiz Question Pool 8 Spring 2008 Below please find the eighth of the weekly lab quizzes. Your T.A. will choose 5 points from those listed below and will write another 5 points worth of questions. The total point value for each quiz will be 10 points. 1) What is the overall goal for this lab? (2 points) 2) Molecular biologists use a number of normal cellular enzymes to manipulate genes. 3) Why must plasmids have selectable markers? (1 point per reason up to 2 points) 4) Give two similarities between plasmids & bacterial chromosomes (besides the fact that they’re both DNA). (2 points) 5) a. What is the doubling period of E. coli under optimum conditions? (1 point) b. If you start a culture at 5 PM with one E. coli cell, how many will you have at 8 AM the following morning, assuming that the bacteria experience optimum conditions all night? Show your work! (2 points)
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Unformatted text preview: c. In fact, bacteria use up all the nutrients in standard medium by the time they reach a density of about 10 10 cell/ml. In order to feed all the bacteria in part (b), how many liters of medium would you need? To put this in perspective, remember 1 liter is about 1 quart! (2 points) 6) a. What do restriction enzymes do? Include some detail! (3 points) b. Why do bacteria make restriction enzymes? (2 points) 7) Draw a picture which summarizes ligating a gene into a plasmid. (3 points) 8) What are 2 strategies to prevent a linearized vector from closing on itself during a ligation? (2 points) 9) What is transformation? (2 points) 10) a. In order to do a blue/white screen, where must the 2 parts of the lacZ (β-galactosidase) gene be? (2 points) b. If both parts are present & functional inside an E. coli cell, what color will a colony grown from that cell on X-gal be? (1 point)...
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This lab report was uploaded on 04/04/2008 for the course BIO 2203 taught by Professor Athwal/haines during the Spring '08 term at Temple.

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