LECTURE1Bdunn

LECTURE1Bdunn - Control of bacterial gene expression, the...

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Control of bacterial gene expression, the case of the lac operon Reading: Ch10; 351-367 Optional reading 368-379
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Key elements that control transcription in prokaryotes: Activators and Repressors Figure 10-2
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Allosteric effectors bind to regulatory proteins Figure 10-3
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Repressor protein controls the lac operon Figure 10-4
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Lactose is broken down into two sugars Figure 10-5
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Figure 10-6 step 1 The lac operon is transcribed only in the presence of lactose
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Figure 10-6 step 2 The lac operon is transcribed only in the presence of lactose
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Figure 10-6 step 3 The lac operon is transcribed only in the presence of lactose
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Figure 10-6 step 4 The lac operon is transcribed only in the presence of lactose
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Structure of IPTG Figure 10-7 IPTG good experimental inducer of lac operon IPTG or lactose both bind to the allosteric site on lac repressor IPTG is not degraded by ß- galactosidase enzyme, so it is stable inducer
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Discovery of the Lac system: Jacob and Monod Frst observed that ß-galactosidase, lac permease and transacetylase enzymes were all induced together. Also, these three genes mapped very close together by recombination mapping. This suggested some kind of common regulatory mechanism for all three genes. To uncover the regulatory mechanism, they made use of mutations in both the genes themselves and mutations in the regulatory elements to deduce operon regulation ±or many of the key experiments, they used partial diploids of the lac gene region by creating ±’lac plasmids (± factors with lac genes)
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Figure 5-18 Faulty outlooping produces F´, an F plasmid that contains chromosomal DNA Cells which have F’ factors are partially diploid.
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This note was uploaded on 12/21/2010 for the course BIOL BIOL 202 taught by Professor D.schoen during the Summer '08 term at McGill.

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LECTURE1Bdunn - Control of bacterial gene expression, the...

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