LECTURE2Adunn

LECTURE2Adunn - Lecture 2A: Regulation of genes in...

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Unformatted text preview: Lecture 2A: Regulation of genes in eukaryotes Some lessons from the simple yeast Reading: Chapter 11; 385-401 Lecture 3: Transcriptional regulation in eukaryotes Review of information from Biol 200 Read Chapter 8 including:- mechanism for transcription- RNA polymerase II- General Transcription factors- Initiation of transcription, TATA box etc- RNA processing including RNA splicing, CAP modiFcations, poly A addition Overview of transcriptional regulation Figure 11-2 General transcription factors Bind to core promoter involved in recruiting RNA pol II TFIID TATA binding protein (TBP) & associated TFs Leads to recruitment of other general TFs + RNA pol II holoenzyme Review Ch 8: Transcription Initiation General transcription factors Bind to core promoter involved in recruiting RNA pol II TFIID TATA binding protein (TBP) & associated TFs Leads to recruitment of other general TFs + RNA pol II holoenzyme Review Ch 8: Transcription Initiation Fig 8-12 Promoter-proximal elements precede the promoter of a eukaryotic gene up to -200 bps. Proximal elements bind factors that act at many genes. Figure 11-3 Single nucleotide substitutions show that the promoter-proximal elements are necessary for efficient transcription Figure 11-4 Model Organism budding yeast Saccharomyces cerevisiae Bakers yeast Model Organism: Yeast The Gal pathway: Yeast cells can break down the sugar galactose to glucose-1-P for energy and carbon metabolism. Like the lac operon of E. coli, yeast cells only produce the enzymes for galactose metabolism when galactose is present. Four enzymes required, each synthesized by a separate gene. Gal enzyme genes are not clustered into an operon, but are separated and located on two different chromosomes....
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This note was uploaded on 12/21/2010 for the course BIOL BIOL 202 taught by Professor D.schoen during the Summer '08 term at McGill.

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LECTURE2Adunn - Lecture 2A: Regulation of genes in...

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