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Unformatted text preview: Full Review Sheet (5-6) Monday, June 02, 2008 9:20 PM Lecture 5 Genomics Genomics - the subdiscipline of mapping, sequencing, and analyzing the function of entire genomes C-Value Paradox - the complexity of an organism is not proportional to the size of the physical genome Southern Blotting - technique to separate and analyze DNA through the use of VNTR (Variable number terminal repeats) Northern Blotting - technique to separate and analyze mRNA Western Blotting - technique to separate proteins by size and charge, then probed with an anti-body Chromosome Walking - when a known location is not too far away from the gene of interest, chromosome walking is a way to slowly move through DNA, one known restriction site at a time, until you reach the gene of interest. First, you pick a known location and find a clone within a gene library that carries that known sequence. The clone will naturally be extended from that sequence on both sides, going to one side will lead you closer to your gene of interest, and going to the other will lead you further. By cutting the clone with Restriction enzyme 1, restriction enzyme 2, and then both restriction enzymes, you can see which restriction site is closer to your gene of interest than your original known gene. Picking this spot as the next beginning point, you repeat the process. Slowly, you gather overlapping clones that eventually lead you to your target gene. (again, textbook explanation is much better). Contig map - a collection of many overlapping clones that, in combination, sequence an entire...
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This note was uploaded on 12/21/2010 for the course BIOL BIOL 202 taught by Professor D.schoen during the Spring '08 term at McGill.
- Spring '08