AP Bio Prelab 12

AP Bio Prelab 12 - thyl Group while Chlorophyll b possesses...

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ethyl Group, while Chlorophyll b possesses a Carbonyl (Aldehyde) Group (does not contain a Hydroxyl Group) Lutein (contains a Hydroxyl Group)
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Procedure Part I: 1) Obtain a 25 cm strip of Chromatography Paper a. Use a Pencil to draw a horizontal line 3 cm from the bottom 2) Use a Capillary Pipette to place Plant Extract on the Baseline 3) Repeat Step 2 until a dark streak of plant extract is visible a. Allow paper to dry between each repeat and keep the streak narrow 4) Obtain a Chromatography Development Jar- 100 mL graduated cylinder w/ 15 mL of Chromatography Solvent (petroleum ether and acetone mixture) a. Seal the jar with a rubber stopper 5) Use a thumb tack to hang the Chromatograph Paper from the rubber stopper with the bottom just touching the solvent in the cylinder, and the Pigment Strip above the solvent 6) Wait 15-20 minutes for the solvent to reach about 1 cm from the top of the paper, then remove the paper and mark the location of the solvent with the pencil a. Label the Pigment Spots 1-4 from top to bottom b. Measure the distance traveled by each pigment from their origin in millimeters to the 10 th s place Results Part I: Calculate the Rf (Rate of Flow) value for each pigment Rate of Flow = (Distance Pigment Migrated) / (Distance Solvent Migrated) **Note: Rate of Flow has no units since it is distance/distance** Migration of Various Photosynthetic Pigments of Spinach Leaves Spot # Pigment Color
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AP Bio Prelab 12 - thyl Group while Chlorophyll b possesses...

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