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Unformatted text preview: Gene regulation Know the function of the promoter and operator in an operon. Are they part of the protein or DNA? What it meant when we say the operon is On? How does the tryptophan operon work? How does the lac operon work? Mutations What are silent, missense, nonsense and frameshift mutations? How can they affect protein function? Gene transfer Briefly describe the major differences among transformation, conjugation and transduction. (players involved, how DNA is transferred) Chapter 9 Describe how gene of interest ends up as a biotech product. Know how Southern Blots work and what they are used for. Given a DNA fingerprinting, be able to identify which 2 microorganism are the same. Know how PCR works and the reagents that are required. Definitions to know: 1 Operon Codon Transcription Translation Replication 2...
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