f04_exam_3_key - Nov Gagao 04:46P p.1 ‘1: O (i , €¥...

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Unformatted text preview: Nov Gagao 04:46P p.1 ‘1: O (i , €¥ Cum?) 1' (189m) U ' th d' tth left f 11' the m t boxes and answer the lucose mp sing 'e 1agrarna e , 1 in I e py v g ’37 followmg questions. Answer NONE if that is an appropriate answer: * ligmp (1 pts each) glucose-6—P a. step(s) are regulated \ 1 g! i O _. +3J’L “"90 b. Which step(s) generate ATP 7) '0 Jrth -a t. bt‘: tw‘: ha 44v 44' 0. Which step(s) consume ATP 1 E 3) glyceraldehyde 5 dihydroxyacetone guts .3 -3eP “m; (- phosphate d. What is the name of the enzyme for reaction 7 aLLt‘moq. 6¢C7Nmu h 05 rock; ‘nase 1,3 bisphosphogl‘yferate a 2. 3 i5" Wit? AT?) e. Which step uses a thioester enzyme intermediate ,2 3-P glycerate f. Production of lactate provides NAD+ for which step(s) in st anaerobic glycolysis t phosphoenolpyruv t6 g. Which step uses aphospho—histidine intermediate 8 at 104C ‘ T? _ . . h. (10-13) Which step produces an mtermedlate for the synthe51s of ‘ 11 Jr the allosteric regulator of hemoglobin? Q 1 tt ac a e i. Which intermediate(s) is(are) a product(s) of the pentose phosphate pathway? Write out names. today. » La — th-e ' . 2. 5 pts (9-12) Given the following reaction mechanisms: 21. Glutamate,j;_1§y3 6m"? glutamine +h ATP <——> ADPTPPv-m ' _ b. Glu + TP 6—9 lutamylph ADP "\x glutamylphosp a + H20 + NH; (---9 glutamine + P,- ‘x W" Which reaction mechanism has a common high energy intermediate in both reactions, A or B 6 ? \ ircle the high energy intermediate above. 1 {yr [I \ Which reaction mechanism has a reaction that dissipates heat, A or B A ? Circle the one~—/ reaction above that dissipates the heat. PT I Which is the more likely reaction mechanism, A e. g ' .7 Lch Rectum» UOKU‘x {EDNA teem. fiwifl M @RWWML "is new; DALE HOV 03—20 0"}: 46F 3. 10 pts (10-23) Use the list to answer the following (#of incorrect answers will be subtracted from # of correct answers). An answer may be used more than once and each question may have more than one answer. a. (10—23) What are the DIRECT fates of glucose—6-phosphate in a liver cell? 0. C 9 CL 3, muscle cell? 0.; e3 2, mogu‘ Cg Mar c. Which metabolic pathway produces NADPH? G, d. Which metabolic pathway produces ribose-S-phosphate? e. Which pathway uses FMN ? a 4. 4 pts (10—13) You will recall that 2,3 bis phosphoglycerate (BPG) lowers the affinity of hemoglobin for oxygen. BPG is synthesized from a glycolytic intermediate. Deficiencies in glycolytic enzymes in erythrocytes have effects on oxygen binding of hemoglobin. Curve B represents normal hemoglobin. 3P“? Which curve represents a deficiency in hexolginaéé D ‘l Which curve represents a deficiency in pyruvate kinase? 3: 6'" a (3&1 Lola} he V MLmMcrtach (YDTh‘eC-‘lfi—dif'iw d Up‘ 30 902%”) A.” '~ ‘ , a l , . Q a g .0: rflndfi £5 215:9 9 low 0 .4 .. w. é *1 a “66"” 2% g 3 * “' vi «3*; E3206: limit t! a a a it; waif.» hrirtit‘i “"4. ill” I" a “V a] ‘ J I. i Nov Gagao 04:46P i be 0 \ P L 5. (20 pts.) Using the diagram on the right, fill in the missing information and anSWer the following questions. Answer NONE if that is an appropriate answer. Assume the cycle only goes in the forward direction. (1 pts each) a.Which step(s) produce FAD (Q) None ___.—-— b.Which step(s) consume NAD+ 3a 4, 3' c.Which reaction(s) use TPP as a cofactor d.Which step(s) release C02 3, El 7 e. Which step(s) directly generates a high energy phosphate bond 5 f.What is the immediate precursor for compound * [2%[0 V0.1!) g. Which step(s) use an intermediate with a thioester bond I i h. What is the name of the enzyme for step 7? maniac» i. (9—7) Under standard conditions (NOT cellular) which direction does reaction 2 prefer?_b_¢LCEAUCL/r (£3 4’2) 74‘ 7...». “Ufa4 hart, fit / + exudate - ' .1 .s - was. 83 MAN? \ 13 U L5“ malate Md citrate 24v - 61C?» Succinate you?“ w or—ketoglutarate tail“ 0%, hw(355 w ( gin“? ‘c I. Li asp NAM 7"”) 96“ j. A deficiency of a citric acid cycle enzyme results in severe neurological abnormalities in newborns. The basis of diagnosis of this metabolic disorder is the presence of abnormally high amount 5 of or- ketoglutarate and succinate and the product of reaction 6. The enzyme most likely to be absent or contain a mutation in the active site lowering its activity is Mr (we) (give name). k. Which reactions have enzymes with an active site histldine? la 5 i - be US. 21 Olmwi‘i‘r “0+ fl 6. ( 5 pts) Arrange the following reactions of the pyruvate dehydrogenase complex (PDC) in the order they occur. FADE-I2 + NADf ——> FAD + NADH + H‘ /5—acetyl ,SH + CoA-SH ——> acetyl-CoA + Diaz‘s“ Lipwsr—r Pyruvatc + TPP thdroxyethyl—TPP + COz /5H ,s LipnsH + FAD —» up“; + PADHZ S /S-acctyl VHydroxyethyl—TPP + Lip1:1s —’ TPP + LiPthH 3 a HOV 03—20 0"}: 43F Wk WU) 7. (12 pts) Identifl the coenzyme, CIRCLE the region that participates in the chemical reaction and name the t e of reaction is articiates int CIRCLE TI-IE PART THAT DOES THE CHEMISTRYIE NAME CH 0 \2 t - // CH -—~ (,Hz— CI-IZWCI-Iz— Cl-l r C\ / \ OO—-l'#() ‘ 1': n l 171 ’w‘ a t 2 . (a) on 0H (30 —~ I’ w. n i i OH CH 7. l E i it Y m I (in o o I ll H ' H‘c (Cl-i3- CH5~0 r-m-aéo w 1’ -— 03 I A_m__ l I Mi2 4. , _ be (130 ' ® - I t N/ filly—TV - . A I HAD 'N (n rt «:11: Q L} H is 1 ‘1 ii ' C” r FIL—N' '-C'—'€'II,V*E'}I;* N" "(,'-- \‘ITI "" I" "-f'iir'ei) “ ‘ P film‘- I’ "H‘l'tAL‘Hr ’ ‘ It It ‘ 3 ' ; L 1 ml r H, Wu‘m nu 8. 4 pts Multiple choice. Circle the best answer. A 4 ‘7 w \ i\ a “A ‘ El What are the net reactions of Complex 1? a: x ‘ “52* A) electrons are transferred to ubiquinone (Q) \/ J “as: \ " A Q B) ATP is synthesized from ADP‘I‘ “‘2 fl? WWW , I \t ‘5’" C) four protons are translocated to the intermembrane spacev’ J 5; D AandB AandC Nov 02—20 04:4SP 7 p.5 .1. reduced cofactors are obtained from the citric acid cycle? NADH and QHZ x/ B) NAD+ and QHZ C) NAD” and NADP+ D) all of the above E) none of the above Which of the following are true statements about the structure of ATP synthase? A) it has a membrane embedded component called F0 V B) it has a component found in the matrix referred to as F1 \/ C it has a trimer of cytochrome subunits 7s (9)- and B A, B, and C How is oxidative phosphorylation regulated? A) by the availability of reduced cofactors v B) by a mitochondrial protein that binds to ATP synthase v C) by the availability of ADP and P,- 1/ D) A and C ® A, B, and C . U" 9. (8 pts) True/False l 9‘ Lo 33L P Co—enzyme Q gathers electrons from NADH and FADHZ and delivers them to complem. T Complex It, also known as succinate dehydrogenase, translocates no protons. P_Electrons follow the same pathway in the Q-cycle) J4. 2 claustrme NEW 9 Wm“ Own“ 1 The chemiosmotic theory suggests that the proton-translocating activity of the electron transport chain generates a proton gradient, and that this source of potential energy is used to synthesize ATP. ‘ 'prolon grad. 01+ 1 Thermogenesis is a process that allows organisms to generate heat by disruption of We. Metabolites in rat muscle were measured before and after exercise. An increase in oxaloacetate and a decrease in PEP were measured. Pyruvate concentrations were unchanged. Answer the questions below by circling the correct answer. Decrease in PEP due to need for ATP®Jr False) Increase in oxaloacetate due to carboxylation of pyruvater False) The rate of consulition and production of pyruvate are equal due to need to make Wm (True I w 01¢:inle Nov 02—20 04:4SP 7 p.6 10. (Problem 12.14) 6 pts The sequence of events in electron transport was elucidated in part by the use of inhibitors that block electron transfer at specific points along the chain. For example, adding rotenone (a plant toxin) or amytal (a barbiturate) blocks electron transport in Complex I; antimycin A (an antibiotic) blocks electron transport in Complex 111; and cyanide (CN') blocks electron transport in Complex IV by binding to the Fe2+ in the Fe-Cu binuclear center. a. What happens to oxygen consumption if any one of these inhibitors are added to a suspension of respiring mitochondria? Circle one. é "' "" on . Pbiofli GT) awnW-V gut Jo 02 J4: moth L130 b. What is the redox state of the electron carriers in the electron transport chain ifrotenone, antimycin or cyanide are added separately to the mitochondrial suspension? Circle the redox state for each component of ET in the presence of the inhibitor indicated. , ,t ,r— ox m as) roman mar m 2". _ at we re:- e c. What is the effect of added succinate to rotenone-blocked, antimycin—blocked, or cyanide-blocked mitochondria? In other words, can succinate help "bypass" the block? Circle the correct answer. i , a me— at!“ n: cyanide % a; E- EJ ...
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This note was uploaded on 12/30/2010 for the course CH 369 taught by Professor Kbrowning during the Spring '07 term at University of Texas at Austin.

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f04_exam_3_key - Nov Gagao 04:46P p.1 ‘1: O (i , €¥...

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