_Dr. Miller's lecture_chemokine_lecture_3

_Dr. Miller's lecture_chemokine_lecture_3 - III. Chemokine...

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III. Chemokine Functions III. Chemokine Functions In Vivo In Vivo A. A. Overview Overview Approaches to study chemokine function Approaches to study chemokine function in in vivo vivo B. B. Constitutive Constitutive Role of chemokines for lymphocyte Role of chemokines for lymphocyte organization within lymph nodes organization within lymph nodes C. C. Inflammation Inflammation Role of chemokines for induction of allergic Role of chemokines for induction of allergic responses responses
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The role of CCR7 in Th1 and Th2 cell localization within secondary lymphoid organs Differentiated CD4+ T helper cells differ in their ability to provide B cell help Th2 cells efficiently provide B cell help, promoting the production of IgG1 and IgE by immunoglobulin class switching Th1 cells are very inefficient at providing B cell help, although can induce class switching to IgG2a B. B. Constitutive Constitutive Role of chemokines for lymphocyte organization within lymph Role of chemokines for lymphocyte organization within lymph nodes nodes
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Hypothesis : Based on what is known about the function of these subsets, the investigators hypothesized that the Th1 and Th2 cell subsets traffic to different microanatomic locations within secondary lymphoid tissues
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Experiment: 1. Isolated splenocytes from a transgenic strain of mouse generated to carry a class II restricted T cell receptor for ovalbumin (DO11.10) 2. Generated Th1 and Th2 cells in vitro 3 . Injected naïve, Th1 or Th2 cells into wild type mice and localized their distribution within the spleen and lymph nodes using an antibody that recognizes the transgenic mouse strain
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Localization patterns of CD4+ T lymphocytes within mouse spleen Th1 Th2 naive
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mRNA analysis of the isolated Th1 and Th2
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_Dr. Miller's lecture_chemokine_lecture_3 - III. Chemokine...

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