2005mcb200f1 - University of Cape Town Department of...

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University of Cape Town Department of Molecular & Cell Biology Course Code: MCB200F Course Name: Intro to Biochemistry Paper: 1 Date: 10 June 2005 No.of pages: 14 Marks:130 Time: 3 Hours Venue: DO 1 - 09h00 Attachments: 2 pages & graph paper ANSWER ALL THE QUESTIONS In the interest of economy please write on both sides of the exam script Please answer each section in a different answer book. Section 1 Dr Jerry Rodrigues 71 Marks MCB200F June Examination 2005 Section A (Amino acids and proteins – 28 marks) 1. Shown below is the curve for 50 mL of an amino acid solution titrated with 1.0 M NaOH solution starting from the zwitterionic form of the amino acid . (a) Mark clearly on the graph the position of the pI, the pKa values and the equivalence points. [5] (b) What amino acid was titrated? [1] (c) Calculate the concentration of the amino acid in the solution. [2] (8) 2. Offer a reasonable explanation as to why valine and isoleucine occur more frequently in β -strands than in α -helices. (2) 1
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Questions 3 to 7 are of the multiple choice type (select the best answer). 3. Identify the peptide with the highest extinction coefficient at 280nm, ie. the one that would absorb the greatest amount of UV radiation at a wavelength of 280 nm assuming equal concentration. A. Y-E-D-R-F-W B. R-N-Y-F C. Y-H-L-I-V-L-H-I D. G-G-A-F E. They would all absorb the same amount of UV radiation at 280 nm. (1)
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