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Unformatted text preview: s Primers s DNA Sequencing Related learning objectives s Describe the relationship between the length of a DNA double helix and its temperature stability and relate this to the specificity of binding of an oligonucleotide (a primer) to a DNA molecule. s Describe the properties of DNA polymerases that help in DNA sequencing. s Indicate where to place primers for DNA sequencing given a diagram. s Indicate the role of di-deoxynucleotides that are tagged with fluorescent dyes in DNA sequencing. Biology 112: Unicellular life. Drs. Kion, Spiegelman, Taylor s Indicate the role of electrophoretic separation of DNA in DNA sequencing. s Describe the process of polymerase chain reaction amplification of DNA including the role of each step. s Indicate why the amplification is specific and where primers should be placed to amplify a given region of DNA. Follow-up practice questions Available online by Oct 24 th...
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This note was uploaded on 01/06/2011 for the course BIO 112 taught by Professor Taylor during the Fall '09 term at The University of British Columbia.
- Fall '09