week 7 objectives - s Primers s DNA Sequencing Related...

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Biology 112: Unicellular life. Drs. Kion, Spiegelman, Taylor Week 7 Readings Freeman 19.2-19.3, 20.3 Note: the readings from Week 6 will be discussed this week as well. Online Pre-Class Assignment - Week 7 Due by 8am on Oct 19 th PeerWise question submission on Week 7 material Group A – due by 8am on Oct 26 th PeerWise question feedback All students must answer at least one question and leave feedback during Week 7 Oct 19 th Invention Activity Oct 21 st Eukaryotic Genomes Discussion topics: s Genome structure s Repeated sequences s Multiple chromosomes Related learning objectives: Note: the objectives from Week 6 are also relevant for today. s Describe the structure of SINES, LINES and transposons and s Explain the importance of SINES, LINES, and transposons to eukaryotic gene structure. s Provide a general description of how LINES and SINES accumulate in the genome of eukaryotes. Oct 23 rd PCR and Forensics Discussion topics: s PCR
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Unformatted text preview: s Primers s DNA Sequencing Related learning objectives s Describe the relationship between the length of a DNA double helix and its temperature stability and relate this to the specificity of binding of an oligonucleotide (a primer) to a DNA molecule. s Describe the properties of DNA polymerases that help in DNA sequencing. s Indicate where to place primers for DNA sequencing given a diagram. s Indicate the role of di-deoxynucleotides that are tagged with fluorescent dyes in DNA sequencing. Biology 112: Unicellular life. Drs. Kion, Spiegelman, Taylor s Indicate the role of electrophoretic separation of DNA in DNA sequencing. s Describe the process of polymerase chain reaction amplification of DNA including the role of each step. s Indicate why the amplification is specific and where primers should be placed to amplify a given region of DNA. Follow-up practice questions Available online by Oct 24 th...
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This note was uploaded on 01/06/2011 for the course BIO 112 taught by Professor Taylor during the Fall '09 term at The University of British Columbia.

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week 7 objectives - s Primers s DNA Sequencing Related...

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