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Unformatted text preview: s Restriction digestion s Gene insertion and ligation s Insertional gene inactivation s Finding mutants on plates Related learning objectives: s Define the characteristics or restriction endonuclease enzymes: what they do, how frequently they cut DNA, and be able to predict the results of restriction endonuclease treatment of DNA given simple cases. s Define the reaction carried out by DNA ligase and how it is used. s Outline the process of creating a recombinant DNA molecule from a DNA and a plasmid. s Describe the process of introducing DNA into bacterial cells by electroporation. s Describe the process of finding bacterial cells carrying Biology 112: Unicellular life. Drs. Kion, Spiegelman, Taylor recombinant plasmids using plating, in particular using insertional inactivation of the lacZ gene. Follow-up practice questions Available online by Nov 14 th...
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- Fall '09