week 10 objectives

week 10 objectives - s Restriction digestion s Gene...

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Biology 112: Unicellular life. Drs. Kion, Spiegelman, Taylor Week 10 Readings Freeman 19.1, and page 256 Brock 11.3, 11.12-11.14 (e-text chapter 8) Online Pre-Class Assignment – Week 10 Due by 8am on Nov 9 th PeerWise question submission on Week 10 material None (and there was much rejoicing…yayyy) PeerWise question feedback None (good luck on your chemistry midterm!) Nov 9 th Plasmids and Conjugation Discussion topics: s Plasmids s Mechanism of conjugation s Antibiotic resistance s Experiments to test for conjugation Related learning objectives: s Describe the stages of conjugation and the role of the proteins involved in the process. s Describe the features of bacterial plasmids. s Explain the difference between conjugative, mobile and non- mobile plasmids. s Predict the outcomes of simple examples of conjugation experiments. Nov 11 th No Class (Remembrance Day) Nov 13 th Cloning Discussion topics:
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Unformatted text preview: s Restriction digestion s Gene insertion and ligation s Insertional gene inactivation s Finding mutants on plates Related learning objectives: s Define the characteristics or restriction endonuclease enzymes: what they do, how frequently they cut DNA, and be able to predict the results of restriction endonuclease treatment of DNA given simple cases. s Define the reaction carried out by DNA ligase and how it is used. s Outline the process of creating a recombinant DNA molecule from a DNA and a plasmid. s Describe the process of introducing DNA into bacterial cells by electroporation. s Describe the process of finding bacterial cells carrying Biology 112: Unicellular life. Drs. Kion, Spiegelman, Taylor recombinant plasmids using plating, in particular using insertional inactivation of the lacZ gene. Follow-up practice questions Available online by Nov 14 th...
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week 10 objectives - s Restriction digestion s Gene...

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