Kinetics Glucose - Kinetics Glucose Chem 223 Why Most-determined compound in medicine 10 of class will contract diabetes Direct near-IR

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
Kinetics Glucose Chem 223
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Why? Most-determined compound in medicine 10% of class will contract diabetes Direct near-IR spectrometric methods for glucose unreliable “Test strip” methods use chemistry similar to lab (but faster)
Background image of page 2
Why Kinetics vs. Equilibrium Shorter analysis time Selectivity (sometimes) Fewer problems with backgrounds In some cases, directly measure property of interest (catalytic ability) rather than concentration In our case, enzyme is selective for single substrate (glucose) However, HRP is promiscuous for oxidizing organics
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
How Fast Is a Reaction? • Diffusion limitation in H 2 O ~ 10 10 M -1 s -1 H + + OH - →H 2 O Steric and energetic factors slow reaction D: Diffusion coefficient k: Boltzmann constant, 1.38 × 10 -23 J K -1 E a : Activation Energy T: Temperature A: Arrhenius factor ] OH ][ H [ ] O H [ 2 2 - + = k dt d kT E a ADe k - = 2
Background image of page 4
Common Rate Laws First Order Second Order Michaelis-Menten P R P R R + 2
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 6
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 01/06/2011 for the course CHEM 223 taught by Professor Scheeline during the Fall '08 term at University of Illinois at Urbana–Champaign.

Page1 / 11

Kinetics Glucose - Kinetics Glucose Chem 223 Why Most-determined compound in medicine 10 of class will contract diabetes Direct near-IR

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online