Spectrophotometric Working Curves

Spectrophotometric Working Curves -...

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Spectrophotometric Working  Curves
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Main Concepts Precision depends on all aspects of an  experiment A linear relationship between an observable  and concentration is convenient Convenience and reality do not always  correspond When Signal = constant * concentration +  offset, finding concentration of an unknown is  simple AND determination of the precision  with which we know concentration is simple
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Transmission: Measureable I 0 Illuminate with I 0 , observe I 1 or I 2 or … I 1 I 2 How does  I 1  compare to  I 0 ? Depends on thickness, wavelength, concentration, … 
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If  I 1  =  I 0 /2 then… I 0 I 1 I 2 I 2  =  I 1 /2 and  I 2  =  I 0 /4. I/I 0  =  T  = Transmittance.  0 to 1 OR 0 to 100%.
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Intensity is observed. TWO intensity measurements are required  to make ONE transmittance measurement. If 
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Spectrophotometric Working Curves -...

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