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Quiz7 - AnswerKey PostlabQuiz FirstWeekofCoNiLab...

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Answer Key Post lab Quiz First Week of Co Ni Lab Chemistry 223 Fall, 2010 1. Why was it necessary to pour the column all at once rather than pouring small amounts of stationary phase into the column in increments? (1) This prevents column cracking. Cracks would allow analyte through the column without separation, defeating the purpose of doing chromatography. 2. If, instead of keeping the liquid/mobile phase and sample as just a thin layer at the top of the column you added the sample to the column when there was a 2 cm layer of mobile phase at the top of the column, what problem would you expect to have? (1) The peak would be broadened, so separation would take longer and the sample would end up more
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