exam_1(3) - Name: _ 7.06 Cell Biology QUIZ #1 This is an...

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Name: ______________________________ 7.06 Cell Biology QUIZ #1 This is an open book exam, and you are allowed access to books and notes, but not computers or any other types of electronic devices. Please write your answers to questions in pen (not pencil) in the space allotted. Please write only on the FRONT SIDE of each sheet. And be sure to put your name on each page in case they become separated. There are 10 pages to the quiz, make sure you have a complete copy! Remember that we will Xerox all of the quizzes. Good Luck! Question 1. 30 pts ________ Question 2. 20 pts ________ Question 3. 30 pts ________ Question 4. 20 pts ________ 1
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Question 1 (30 points) Name: ______________________________ You are studying activated T helper cells in a mouse model system. T helper cells are a sub-group of lymphocytes that play an important role in establishing and maximizing the capabilities of the immune system without themselves having any cytotoxic or phagocytic activity. Your lab studies small molecules secreted by T helper cells that activate Natural Killer (NK) cells. A (2 points). You identify a small glycoprotein Interleukin-79 that is secreted by T helper cells, and seems to activate NK cells. This glycoprotein is particularly highly expressed in a subset of T helper cells which you have purified from primary mouse serum. Why isn’t this primary cell line suitable for long term study? B (8 points). You now have found the means to generate an immortal T helper cell line by transformation with a tumor cell virus. You want to find the affinity of Interleukin-79 for its unknown receptor on NK cells. Describe how you would perform a binding experiment to determine this. Include a description of how you would control for nonspecific binding. (Assume 1:1 binding ratio of Interleukin -79 to its receptor on NK cells) 2
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Name: ______________________________ C (4 points). You perform a binding assay and plot your data on a Scatchard plot, which is presented below. Label the x and y axes in terms of [R], [L] and [RL], where R is the receptor, L is the ligand and RL is the receptor-ligand complex. Also, indicate how you would determine the binding constant, K, and the number of ligand binding sites, remembering the equation y= mx + b. 0 0.5 1 1.5 2 2.5 024681 0 1 2 1 4 D (4 points). You isolate a cDNA that you think might encode your receptor. You transfect this into a cell line that does not express immune cell receptors and see no binding. Explain how the composition of receptor in terms of its subunit structure might affect ligand recognition. What experiment might you use to prove your contention?
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exam_1(3) - Name: _ 7.06 Cell Biology QUIZ #1 This is an...

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