exam_2 - Name:_ TA Name and Recitation Time: _ 7.06 Cell...

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Name:__________________________ TA Name and Recitation Time: ____________________________________ 7.06 Cell Biology QUIZ #2 This is an open book exam, and you are allowed access to books and notes, but not computers or any other types of electronic devices. Please write your answers to questions in pen (not pencil) in the space allotted. Please write only on the FRONT SIDE of each sheet. And be sure to put your name on each page in case they become separated. There are 9 pages to the quiz, make sure you have a complete copy! Remember that we will Xerox all of the quizzes. Good Luck! Question 1. 30 pts ________ Question 2. 30 pts ________ Question 3. 20 pts ________ Question 4. 20 pts ________ 1
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Name:__________________________ Question 1. (30 points) The questions below ask you to evaluate experimental data. 1a. (6 points) You use FACS to measure the DNA content of three samples: a cell preparation from mouse testis with all the testis cell types, isolated prophase I arrested mouse oocytes, and mammalian cell culture. Unfortunately you forgot to label the tubes. Which of the three FACS profiles below is most likely to correspond to each of the three samples? Explain your reasoning. 1b. (6 points) You analyze the kinetics of polymerization of α−β tubulin dimers in vitro. You use turbidity as an assay for polymerization because this increases with polymerization and find the following: 2
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Name:__________________________ If you add γ -tubulin rings you observe the following Explain the effect of γ -tubulin. How does this account for the role of γ -tubulin in vivo and its cellular location? 1c. (10 points) You did an experiment in which you incubated the cohesin complex with Separase alone or Securin and Separase in vitro then ran a gel, prepared a western blot and probed it with an antibody against the Scc1 subunit. In a separate in vitro experiment you incubated CyclinB protein with Cdh1/APC with or without the 26S proteosome, ran a gel, made a western blot and probed it with antibodies against CyclinB. Not having learned from failing to label your tubes in the FACS experiment, you failed to label the
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This note was uploaded on 01/07/2011 for the course BIOLOGY 7.06 taught by Professor Nitro during the Spring '10 term at MIT.

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exam_2 - Name:_ TA Name and Recitation Time: _ 7.06 Cell...

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