exam_4 - Name:_ TA Name and Recitation Time: _ 7.06 Cell...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Name:__________________________ TA Name and Recitation Time: ____________________________________ 7.06 Cell Biology QUIZ #4 This is an open book exam, and you are allowed access to books and notes, but not computers or any other types of electronic devices. Please write your answers to questions in pen (not pencil) in the space allotted. Please write only on the FRONT SIDE of each sheet. And be sure to put your name on each page in case they become separated. There are 10 pages to the quiz, make sure you have a complete copy! Remember that we will Xerox all of the quizzes. Good Luck! Question 1. 25 pts ________ Question 2. 20 pts ________ Question 3. 30 pts ________ Question 4. 25 pts ________ 1
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Name:__________________________ Question 1. (25 points) You are interested in studying actin dynamics. Your advisor gives you purified G actin- ATP monomers and two proteins she is convinced affect actin dynamics. She also provides you with the Myosin S1 protein fragment. 1a. (2 points) Draw the profile you observe when you add an actin nucleator seed of polymerized actin to a G-actin-ATP solution at a concentration above the critical concentration (Cc) for the - end. Assume the time course goes long enough that equilibrium is reached. Filament mass time 1b. (5 points) Draw the profile you observe when you add actin nucleator seed of polymerized actin to a G-actin-ATP solution at a concentration between the Cc for the + end and the Cc for the end. Filament mass time What is the term for what is observed? 2
Background image of page 2
Name:__________________________ 1c. (9 points) When you add Protein X to a reaction with an actin nucleator seed of polymerized actin and G-actin-ATP at a concentration above the critical concentration (Cc) for the end you observe the following: Filament mass Addition of Protein X time Propose two possible functions for Protein X and design an experiment to distinguish these functions. 1d. (3 points) When you add Protein Y you do not observe an effect on filament mass. You show by co-immunoprecipitation that Protein Y will bind to actin microfilaments but
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 10

exam_4 - Name:_ TA Name and Recitation Time: _ 7.06 Cell...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online