pset1(3) - 7.06 Spring 2013 Problem Set 7 Question 1 You...

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7.06 Cell Biology, Spring 2007 Problem Set1 Question 1 a) You are studying a transmembrane protein Tmp1. Your PI wants you to raise a monoclonal antibody against the predicted extracellular domain. Describe how you would do this. 1b) You now have a monoclonal antibody against Tmp1. Describe other tools you need for localization or Western blotting studies. 1c) Describe how you could use this antibody to show that epitope in your predicted extracellular domain is indeed extracellular. 1d) What is another method you could have used to determine the localization of Tmp1 without the use of antibodies? What is a possible caveat to the use of this technique?
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1e) How could you use this alternate technique to assess the mobility of Tmp1 in the membrane? Describe the possible results and their interpretation. Question 2 ) What are the differences between primary cells and cell lines? What are the advantages and disadvantages of each? Question 3
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This note was uploaded on 01/07/2011 for the course BIOLOGY 7.06 taught by Professor Nitro during the Spring '10 term at MIT.

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pset1(3) - 7.06 Spring 2013 Problem Set 7 Question 1 You...

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