Fiehn BIS103 lecture 17_fall 2010

Fiehn BIS103 lecture 17_fall 2010 - Analysis of metabolism

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1 Analysis of metabolism http://pubchem.ncbi.nlm.nih.gov/search/ compound structures, literature http://www.genome.jp/kegg/ligand.html metabolic maps
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2 Phenotype Genotype mRNA expression protein expression metabolite expression mass spectrometry ~ 200,000 compounds (in plant kingdom) ‘Complexity‘ 7 orders of magnitude dynamic range bad genome annotation little knowledge about network regulation diverse chemistry, no amplification
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3 Why measure metabolites ? Simple answer: h readout of the underlying molecular network h infer enzyme activities h next to any observable phenotype: diagnostics, functional genomics…. Not so simple answer: h not victims, but actors h a cause somewhere in the network can have effects elsewhere Metabolomics is a branch of Analytical Biochemistry Analytical biochemistry is the analysis of material samples to gain an understanding of their biochemical composition and structure. Sample (mixture of compounds plus matrix) separation (w/ extraction) Qualitative analysis seeks the presence of a given functional group or organic compound in a sample. Quantitative analysis
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Fiehn BIS103 lecture 17_fall 2010 - Analysis of metabolism

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