Microbial Physiology notes lec16 11-1-10

Microbial Physiology notes lec16 11-1-10 - Microbial Physio...

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Microbial Physio Lecture 16: F-ATPase: A Rotary Motor November 1, 2010 1. 2. Treated the bacteria/mitochondrion w/ certain detergents, and found that the bacteria have ATPases that fall into 2 distinct groups—there are 2 segments of the F-type ATPases (the thing falls apart but only partially because both of the 2 segments contain several of the subunits) a. F 1 subunit: i. Soluble form that exists in the overall structure of the F-type ATPases—localized on the inside of the membrane regardless of what organism the F-type ATPase is in ii. All of the Greek letter subunits (alpha, beta, gamma, delta, epsilon) go to the soluble fraction iii. Stoichiometry of soluble F 0 form: 1. 3 subunits of alpha 2. 3 subunits of beta 3. 1 subunit of gamma 4. 1 subunit of delta 5. 1 subunit of epsilon b. F 0 i. Insoluble form ii. All of the Arabic letters go to the membrane (a,b,c) 1. So with respect to the membrane there are 3 types of subunits(constituents) they’re called a,b,c subunits 2. a subunit is a monomer 3. b subunit is a dimer a. in some bacteria they don’t have 2 identical b’s, but they have a b and a b’ because they have 2 b subunits that have become dissimilar due to and extra-genic duplication event followed by sequence divergence so the 2 B subunits are homologous, but no longer identical 4. c subunit has 12 subunits a. There is no set # of subunits the ring can have anywhere from 8-15 subunits c. Transmembrane subunits: i. a= 5 or 6 TMSs ii. b= 1TMS iii. c= 2TMSs d. F-ATPases
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i. Found in chloroplast, mitochondria, bacteria, archaea ii. They’re virtually ubiquitous iii. The V-type ATPase ( V=vacuolar) in eukaryotes are found w/in the intracellular plasma membrane of eukaryotic cell particularly in the vacuoles, ER, golgi apparatus 1. V-ATPase is an offshoot of the F-type ATPases iv. Archaea have an ATPase also A-type ATPases 3. H + channel a. H + flows through the channel comprised of a subunit and c subunit this is then what produces the torque to cause rotation of the rotor to drive the ATP synthesis 4. ATPases: a. F-type ATPase ~12 c subunits; 2 TMSs; 12 H + /3ATP/rotation i. 1 rotation producing 3 ATP is constant, what differs is the # of protons going into
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Microbial Physiology notes lec16 11-1-10 - Microbial Physio...

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