Microbial Physiology notes lec18 11-5-10

Microbial Physiology notes lec18 11-5-10 - Microbial Physio...

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Microbial Physio Lecture 18: References for H + specific transporters November 5, 2010 1. H+ channels a. Viral H+ channels i. The virus makes the viral channel forming protein that goes through the membrane 2x’s ii. The protein gets protonated and it fxns like a channel that allows the free flow of H+ iii. Why would a virus want to have in its genome a gene that codes for a proton channel? this is true of almost all the envelop viruses 1. the intracellular vesicle in which the virus particle binds itself, and it binds itself because the phagocytosis mechanism of the host cell has taken the virus up from the outer surface of the host plasma membrane 2. and then once the virus is enveloped by the host membrane, then virus wants to make the medium acidic because that is what gives rise to activation of the viral DNA 2. H+ Carrier a. Mitochondrial carrier “uncoupling proteins” i. the uncoupling proteins fxn in certain tissues of our bodies, such as lipophilic tissues to allow free flow of H+ across the membrane ii. why would you want to have a free flow of H+ across the membrane because it wastes energy iii. So they fxn in the transport of protons, but its not really the proton that’s transported, they transport a negatively charged species which is a fatty acid—not the whole thing, but the negatively charged carboxyl group at the end of the fatty acid is transported forwards and backwards, and as a result the protons which can attach to the carboxyl group can flow down their [ ] gradient that gives rise to generate heat because allowing H+ to flow down their [ ] gradient into the cell, and consequently you generate heat—impt in warm blooded animals 1. because when you waste energy you release heat the purpose of the free flow of H+ across the membrane is to keep the organism warm 2. mammals are warm blooded, so we keep our temperatures the same always our metabolic activity isn’t sufficient to keep us at the right temperature, so as a result our body has these uncoupling proteins to produce heat, by allowing the free flow of H+ across the membrane combined w/ hydroxyl groups on the other side of the membrane exothermic rxn 3. H+ Pumps a. F-Type ATPase i. Is the rotor of the cell which generates ATP from the pmf, and generates the pmf from the ATP b. P-type ATPase i. 2 families that contain H+ transporters 1. Na+/K+ ATPase a. Transport H+
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i. What they actually do is exchange H+ for K+ ii. Na+/K+ATPase 1. have 3Na+ and 2K+ and you form a membrane potential 2. Only found in 1 part of the animal body you have sequence similar H+/K+ATPase i. Pumps 2H+ out of cell for 2K+ into cell ii. It is the stomach have to have high acidity in stomach to kill bacteria 3. Plant plasma membrane proton pump ATPase a. A highly regulated P-type ATPase with multiple physiological roles b. Found in plants, fungi, and unicellular eukaryotes c. H+-PPase i. Proton pyro-phosphatases 1. this enzyme hydrolyze pyrophosphate 2 phosphates that re
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Microbial Physiology notes lec18 11-5-10 - Microbial Physio...

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