175Key questions revisitedgenome was completed, allowing us to compare theexact position of genes on the genetic map with the cor-responding position of the respective coding sequenceon the linear DNA sequence. Figure 5-34 makes thiscomparison for a segment of both maps. Clearly, thegenetic map is a close match to the physical map.As an aside in closing, it is interesting that many ofthe historical experiments revealing circularity of bacter-ial and plasmid genomes coincided with the publicationand popularization of J. R. R. Tolkien’s The Lord of theRings. Consequently a review of bacterial genetics atthat time led off with the following quotation from thetrilogy:“One Ring to rule them all, One Ring to Fnd them,One Ring to bring them all and in the darkness bind them.”ABCABthrtolJ(toll)popDgprBdnaant*gprA*KJrpsTorfileSlspA012345chlG*dapBcarrimF*mafAkefCfolAapaHorfksgApdxAbrnS*ilvJrimGaradadBleuilvmafB*fruR*ftsM*serR*DABC DBCAIHDEACBDpolBmraA,B*ftslmurEmurFmurG
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