Introduction to Genetic Analysis 165

Introduction to Genetic Analysis 165 - labeled IS 1 between...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
164 Chapter 5 The Genetics of Bacteria and Their Viruses of the bacterial chromosome. These plasmids have been useful as vectors for carrying DNA in sequencing large genomes (see Chapters 11 and 12). R plasmids An alarming property of pathogenic bacteria Frst came to light through studies in Japanese hospitals in the 1950s. Bacterial dysentery is caused by bacteria of the genus Shigella. This bacterium initially was sensitive to a wide array of antibiotics that were used to control the disease. In the Japanese hospitals, however, Shigella isolated from patients with dysentery proved to be simultaneously resis- F 'lac F F 'lac /lac 2 partial diploid 1 Excision Insertion (b) (c) (d) (e) Integrated F factor Hfr chromosome lac 1 1 1 ton IS 1 IS 2 1 2 (a) 1 Figure 5-17 Origin of an F 9 factor. (a) F is inserted in an Hfr strain at a repetitive element
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: labeled IS 1 between the ton and lac 1 alleles. (b) The inserted F factor. (c) Abnormal outlooping by crossing-over with a different element, IS 2 , to include the lac locus. (d) The resulting F 9 lac 1 particle. (e) F 9 lac 1 / lac 2 partial diploid produced by the transfer of the F 9 lac 1 particle to an F 2 lac 2 recipient. [From G. S. Stent and R. Calendar, Molecular Genetics , 2d ed. Copyright 1978 by W. H. Freeman and Company.] MESSAGE The DNA of an F 9 plasmid is part F factor and part bacterial genome. F 9 s transfer rapidly like F, and can be used to establish partial diploids for studies of bacterial dominance and gene interaction. 44200_05_p151-184 3/4/04 10:47 AM Page 164...
View Full Document

This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.

Ask a homework question - tutors are online