Introduction to Genetic Analysis 173

Introduction to Genetic Analysis 173 - 172 Chapter 5 The...

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Unformatted text preview: 172 Chapter 5 The Genetics of Bacteria and Their Viruses Using an extension of this approach, we can esti- mate the size of the piece of host chromosome that a phage can pick up, as in the following type of experi- ment, which uses P1 phage: donor leu thr azi r 9: recipient leu thr azi s In this experiment, P1 phage grown on the leu thr azi r donor strain infect the leu thr azi s recipient strain. The strategy is to select one or more donor markers in the recipient and then test these transductants for the pres- ence of the unselected markers. Results are outlined in Table 5-3. Experiment 1 in Table 5-3 tells us that leu is relatively close to azi and distant from thr, leaving us with two possibilities: or Experiment 2 tells us that leu is closer to thr than azi is, so the map must be By selecting for thr and leu together in the transducing phages in experiment 3, we see that the transduced piece of genetic material never includes the azi locus because the phage head cannot carry a fragment of DNA that big.the phage head cannot carry a fragment of DNA that big....
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