Introduction to Genetic Analysis 178

Introduction to Genetic Analysis 178 - 44200_05_p151-184...

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177 Solved problems 1. Suppose that a cell were unable to carry out general- ized recombination ( rec 2 ). How would this cell be- have as a recipient in generalized and in specialized transduction? First compare each type of transduc- tion and then determine the effect of the rec 2 muta- tion on the inheritance of genes by each process. Solution Generalized transduction entails the incorporation of chromosomal fragments into phage heads, which then infect recipient strains. Fragments of the chromosome are incorporated randomly into phage heads, so any marker on the bacterial host chromosome can be trans- duced to another strain by generalized transduction. In contrast, specialized transduction entails the integra- tion of the phage at a specific point on the chromo- some and the rare incorporation of chromosomal mark- ers near the integration site into the phage genome. Therefore, only those markers that are near the specific integration site of the phage on the host chromosome can be transduced.
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