Introduction to Genetic Analysis 180

Introduction to Genetic Analysis 180 - 44200_05_p151-184...

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179 Problems B ASIC P ROBLEMS 1. Describe the state of the F factor in an Hfr, F 1 , and F 2 strain. 2. How does a culture of F 1 cells transfer markers from the host chromosome to a recipient? 3. With respect to gene transfer and integration of the transferred gene into the recipient genome compare the following: a. Hfr crosses by conjugation and generalized trans- duction. b. F 9 derivatives such as F 9 lac and specialized trans- duction. 4. Why is generalized transduction able to transfer any gene, but specialized transduction is restricted to only a small set? 5. A microbial geneticist isolates a new mutation in E. coli and wishes to map its chromosomal location. She uses interrupted-mating experiments with Hfr strains and generalized-transduction experiments with phage P1. Explain why each technique, by itself, is insuf±cient for accurate mapping. 6. In E. coli, four Hfr strains donate the following markers, shown in the order donated: Strain 1: M Z X W C Strain 2: L A N C W Strain 3: A L B R U Strain 4: Z M U R B All these Hfr strains are derived from the same F 1 strain. What is the order of these markers on the cir-
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This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.

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