Introduction to Genetic Analysis 183

Introduction to Genetic Analysis 183 - 44200_05_p151-184...

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182 Chapter 5 The Genetics of Bacteria and Their Viruses 5. Defne the terms prototroph and auxotroph . 6. Which cultures in this experiment are prototrophic and which are auxotrophic? 7. Given some strains oF unknown genotype regarding thiamine and proline, how would you test their genotypes? Give precise experimental details, including equipment. 8. What kinds oF chemicals are proline and thiamine? Does this matter in this experiment? 9. Draw a schematic diagram showing the Full set oF manipulations perFormed in the experiment. 10. Why do you think the experiment was done? 11. How was it established that pro enters aFter thi ? Give precise experimental steps. 12. In what way does an interrupted-mating experiment diFFer From the experiment described in this problem? 13. What is an exconjugant? How do you think that exconjugants were obtained? (This might involve genes not described in this problem.) 14. When the pro gene is said to enter aFter thi , does it mean the pro allele, the pro 1 allele, either, or both?
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