182Chapter 5 •The Genetics of Bacteria and Their Viruses5. Defne the terms prototrophand auxotroph.6. Which cultures in this experiment are prototrophicand which are auxotrophic?7. Given some strains oF unknown genotype regardingthiamine and proline, how would you test theirgenotypes? Give precise experimental details,including equipment.8. What kinds oF chemicals are proline and thiamine?Does this matter in this experiment?9. Draw a schematic diagram showing the Full set oFmanipulations perFormed in the experiment.10. Why do you think the experiment was done?11. How was it established that proenters aFter thi?Give precise experimental steps.12. In what way does an interrupted-mating experimentdiFFer From the experiment described in thisproblem?13. What is an exconjugant? How do you think thatexconjugants were obtained? (This might involvegenes not described in this problem.)14. When the progene is said to enter aFter thi, does itmean the proallele, the pro1allele, either, or both?
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