Introduction to Genetic Analysis 607

Introduction to Genetic Analysis 607 - 44200_18_p575-610...

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606 Chapter 18 The Genetic Basis of Development for one of three different genes that resulted in embryos that had high, low, or no concentrations of DL protein. Preparations of mRNA from each of these three maternal genotypes were hybridized to microarrays con- taining every gene in the Drosophila genome, and hy- bridization patterns were compared. A total of 40 new genes were found that were activated or repressed by the DL protein, increasing the number of known DL target genes by 500 percent. In follow-up experiments, the research group was able to show that, indeed, these genes were expressed in the early embryo in domains corresponding to the germ layers along the D–V axis (Figure 18-36). In another approach, high throughput two-hybrid analysis has been used to make protein inter- action maps that expand and re±ne the Drosophila body plan networks obtained by mutational analysis. This is just one of many applications of high- throughput techniques for systematically screening an entire genome for genes of interest that can then be in- tensively analyzed to help tease apart an entire develop- mental pathway. This approach does not invalidate the
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This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.

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