Introduction to Genetic Analysis 525

Introduction to Genetic Analysis 525 - 524 Chapter 16...

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Unformatted text preview: 524 Chapter 16 Dissection of Gene Function into the sequence of a gene, it may disrupt the integrity of one of its exons, or its splicing pattern, or its regula- tion, depending on where it lands. As we shall see, trans- posons have an added level of convenience in that if a mutation is induced, the transposon acts as a tag for the mutant allele. This can lead to easy identification of the inserted gene, for example by PCR. The procedure is known as transposon tagging. Screens are harder work but have the advantage of be- ing able to choose a range of phenotypes within a broad general class. Forward genetic selection Microbes are amenable to selections because they can be grown in very large numbers on plates or in test tubes, on media containing specific substances that act as selec- tive agents. These agents will kill all cells except those with the mutations leading to the desired phenotype....
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