Introduction to Genetic Analysis 531

Introduction to Genetic Analysis 531 - 530 Chapter 16 •...

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Unformatted text preview: 530 Chapter 16 • Dissection of Gene Function The geneticist is thus able to focus on those tanks pro- ducing interesting mutations, and the mutants can be recovered by inbreeding the parental F 1 female. Another way to speed up the screen is to use a mo- lecular tag. Earlier we saw that transposons can be used as tags. In zebra fish, retrovirus vectors serve as tags. Re- call that retroviruses are RNA viruses that use reverse transcriptase to create a double-stranded DNA copy of their viral genome. The virus is injected into embryos of size 1000–2000 cells. When retroviral DNA is repli- cated in a cell, the new DNA becomes integrated at ran- dom into the host chromosomes. The retroviral DNA causes mutations by gene disruption, and also serves as a convenient molecular tag for the disrupted genes. The resulting fish are subjected to an inbreeding program as before to bring mutations to homozygosity. Once a mu- tant is identified in a subsequent generation, the gene that it has inserted into can be determined from its se-...
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This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.

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