Introduction to Genetic Analysis 532

Introduction to Genetic Analysis 532 - There are advantages...

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Unformatted text preview: There are advantages to each of these approaches. Random mutagenesis is the easiest to carry out, but it requires time and effort to sift through all the mutations to find the small proportion that includes the gene of interest. Targeted mutagenesis is also labor-intensive, but once the targeted mutation is obtained, it is more straightforward to characterize. Creating phenocopies can be very efficient, but there are limits to the kinds of phenotypes that can be copied. We will consider exam- ples of each of these approaches. Reverse genetics through random mutagenesis Random mutagenesis for reverse genetics employs the same kinds of general mutagens that are used for forward genetics: chemical agents, radiation, or transposable ge- netic elements. However, instead of screening the genome at large for mutations that exert a particular phenotypic effect, reverse genetics focuses in on the gene in question. This can be done in one of two general ways. One approach is to focus in on the map location of the gene. Only mutations falling in the region of the genome where the gene is located are retained for fur- ther detailed molecular analysis. Thus in this approach the recovered mutations must be mapped. One straight-the recovered mutations must be mapped....
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