Introduction to Genetic Analysis 540

Introduction to Genetic Analysis 540 - 539 Key terms •...

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Unformatted text preview: 539 Key terms • What is the purpose of mutational dissection? To find and characterize all or most of the genes that contribute to some biological process of interest. • What are the general strategies of mutational dissection? Start with a collection of mutants obtained using selec- tions or screens and then characterize their wild func- tions at the molecular level (forward genetics); alterna- tively, start with a set of specific DNA sequences, mutate them, and observe the phenotypes (reverse genetics). KEY QUESTIONS REVISITED • How are mutations characterized? They can be classified first as gain of function, loss of function, or new function; then their sequence and cell- expression patterns can be characterized. • Are there alternatives to classical mutagens for dissecting gene function? Yes, there are ways to mimic mutant phenotypes (make phenocopies). This can be achieved by interference with mRNA (RNAi) or by binding highly specific inhibitors to proteins (chemigenomics)....
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This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.

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