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Unformatted text preview: 540 Chapter 16 Dissection of Gene Function SOLVED PROBLEM You want to study the development of the olfactory (smell-reception) system in the mouse. You know that the cells that sense specific chemical odors (odorants) are located in the lining of the nasal passages of the mouse. Describe some approaches for doing both for- ward and reverse genetics to study olfaction. Solution There are many approaches that can be imagined. a. For forward genetics, the first trick is developing an assay system. One such assay system would be behav- ioral. For example, we could identify odorants that ei- ther attracted or repelled wild-type mice. We could then perform a genetic screen after mutagenesis, looking for mice that do not recognize the presence of this odorant, by using a maze in which a stream of air containing the odorant is directed at the mice from one end of the maze. This assay has the advantage of allowing us to process lots of mutagenized mice without any anatomi- cal analysis. As a first step, you might want to identify mutations that act dominantly to affect odorant reception, simply because these mutations could be identified in an F 1 screen. If this didnt work, you might need to resort toscreen....
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This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.
- Spring '08