Introduction to Genetic Analysis 400

Introduction to Genetic Analysis 400 - on File 44200...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
12.4 Using Genomic Sequence to Find a Specific Gene 11 44200 GRIFFITHS FREEM Ch-12 First Pages Sen 12-10-2003 p 11 Application File on File molecular markers and classical phenotypic markers. Molecular markers are DNA polymorphisms, variants in the nucleotide sequence of different normal individual members of a population. DNA polymorphisms can be single nucleotide polymorphisms or variants of large DNA segments. These polymorphisms may be present at 0.01 percent to . 1 percent of the base pairs in the genome, depending on the species and the particular seg- ment of the genome. Thus, the potential density of mo- lecular markers on a linkage map is much higher than that of classical markers. Let’s look at some examples. RESTRICTION FRAGMENT LENGTH POLYMORPHISMS Restriction fragment length polymorphisms (RFLPs) represent the presence or absence of restriction-enzyme recognition sites in different individuals. Any two indi- viduals will differ by a great many RFLPs. An RFLP is as- sayed by using a cloned DNA fragment as a probe. The probe must bind to one site alone, and, in a Southern blot, it will reveal different-sized restriction fragments,
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.

Ask a homework question - tutors are online