Introduction to Genetic Analysis 402

Introduction to Genetic Analysis 402 - on File 44200...

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12.4 Using Genomic Sequence to Find a Specific Gene 13 44200 GRIFFITHS FREEM Ch-12 First Pages Sen 12-10-2003 p 13 Application File on File 5 9 ...... C-A-C-A-C-A-C-A-C-A-C-A-C-A-C-A 3 9 3 9 ...... G-T-G-T- G-T- G-T- G-T- G-T- G-T- G-T 5 9 Variant markers are distinguished by PCR with the use of primers for regions surrounding individual micro- satellite repeats. Cloned DNA containing the microsatel- lite and flanking DNA is sequenced and hence primers can be designed from the abutting regions. An individual primer pair will amplify that micro- satellite locus only because the primers are specific. Any variants of microsatellite size will be revealed by gel electrophoresis of the PCR products from different indi- viduals. Thus, all size variations of the microsatellite re- peat will be detectable. A high proportion of such PCR analyses reveal several marker “alleles,” or different-sized strings of repeats. An example of the microsatellite map- ping technique is shown in Figure 12-12. Thousands of
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This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.

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