44200 16Chapter 12 •Genomics44200 GRIFFITHS FREEM Ch-12 First Pages Sen 12-10-2003 p 16Application FileLinkage mapSequence mapProbe for marker BProbe for marker CProbe for markerAProbe for markerDProbe for markerECytogenetic mapClones ofphysical mapPhysical mapAAGTCTBase changeof marker ABase changeof marker CTGATCCTGAGCDBECFigure 12-17 Uniting linkage andcytogenetic maps with molecularmaps. To connect the different maptypes, molecular markers fromlinkage and cytogenetic maps areanchored onto the physical mapand the genomic sequence map.Why are we interested in such structural rearrange-ments in the context of maps? The reason is that somebreaks cause mutant phenotypes, either because a genethat happens to reside at the breakpoint is deactivated orbecause the junction fuses two genes together, creating anovel gene. Some deletions also cause mutant pheno-types. Hence a link can be made between a mutation anda chromosomal position. How can we map rearrangementbreakpoints relative to molecular markers? One approach
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This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.